Peptide Reconstitution Calculator: BAC Water, Concentration, and Syringe Units

The whole calculation is two lines of arithmetic. Here they are, worked out on a 5 mg vial, plus the places I keep seeing the math go sideways.

LockedIn is a tracking tool, not medical advice. It does not verify what you enter, and it does not recommend doses, schedules, or intake targets. Dose and medication decisions come from your prescriber. Nothing on this page is a substitute for their instructions.

The volume of bacteriostatic water isn't something a calculator decides for you: it comes from your prescriber or from the manufacturer's instructions that came with the product. What the arithmetic does is tell you what any given volume produces. Two lines: milligrams in the vial divided by milliliters of water equals concentration in mg/mL, then (dose in mg divided by mg/mL) times 100 equals units on a U-100 insulin syringe.

Run it on a 5 mg vial with 2 mL of bacteriostatic water. 5 divided by 2 is 2.5, so the vial now holds 2.5 mg per mL. If the dose written on your prescription were 0.5 mg (I'm using that number purely as an arithmetic input, not as a suggestion), then 0.5 divided by 2.5 is 0.2 mL. Multiply by 100 and you get 20 units on a U-100 syringe.

The times-100 step confuses people more than it should. U-100 means the barrel is calibrated at 100 units per milliliter, so one unit is 0.01 mL. Converting mL to units on that barrel is the same move as converting meters to centimeters. If your syringe is marked U-40 instead, the multiplier is 40, not 100, and the numbers on the barrel mean something different.

Everything below is arithmetic and nothing more. I'm a software developer, not a clinician. Dosing, water volume, needles, and injection sites are all outside what I can tell you. Follow your prescriber and the printed instructions that came with your medication, and call them when a number doesn't match what you expected.

Reconstitution calculator

Arithmetic only. The dose comes from your prescriber, not from this page.

The two formulas, written out

Step one gives you concentration. Step two turns a dose into a volume, then into marks on a barrel.

  1. Concentration: vial strength in mg divided by bacteriostatic water in mL = mg/mL.
  2. Volume: dose in mg divided by concentration in mg/mL = mL to draw.
  3. Units: that mL figure times 100 = units on a U-100 syringe.

You can collapse steps two and three into one expression: (dose mg / mg per mL) x 100 = units. That's the version the calculator in my app runs. It's worth understanding the middle step anyway, because the mL number is what you check against the syringe barrel when the unit answer looks strange.

One more definition, since insulin syringes come in a few sizes. A 1 mL barrel goes to 100 units, a 0.5 mL barrel goes to 50 units, and a 0.3 mL barrel goes to 30 units. All three are U-100 as long as they say so on the wrapper. The barrel size caps how much you can draw at once, but it doesn't change the conversion.

A 5 mg vial, four different water volumes

Here's the same vial reconstituted four ways, with the resulting units for a 0.5 mg dose. Again, both the 0.5 mg and the water volumes are arithmetic inputs I picked to show the pattern, not recommendations.

BAC water addedConcentrationVolume for 0.5 mgUnits (U-100)
1 mL5 mg/mL0.10 mL10
2 mL2.5 mg/mL0.20 mL20
2.5 mL2 mg/mL0.25 mL25
5 mL1 mg/mL0.50 mL50

Read across any row and the milligrams delivered are identical. Only the volume changed. This is the single most useful thing to internalize about reconstitution, and it's the point people miss when they see a friend's syringe sitting at a different number than theirs and assume one of them is wrong.

A 10 mg vial behaves the same way with the numbers doubled. 10 mg in 2 mL is 5 mg/mL, and a 0.5 mg dose off that vial is 0.1 mL, or 10 units.

More water doesn't mean more medication

Bacteriostatic water is a carrier. It moves the powder from the bottom of the vial into a liquid you can measure. Adding twice as much doesn't add anything to the vial; it spreads the same milligrams across twice the volume, so every dose sits at twice the unit mark and the vial holds the same number of doses it always did.

Where the volume matters is readability. A dose that lands at 4 units on a barrel gradated every 2 units is a squint. The same dose at 20 units is easy to see and easy to check. That's a real consideration, and it's exactly the kind of thing to raise with the prescriber or pharmacist who set the plan, because they're the ones who can tell you whether a different reconstitution volume is appropriate for what you were given.

Two practical limits on the arithmetic. First, the calculation assumes the final volume equals the water you added. The powder itself occupies a small amount of space, so the true volume can run slightly higher. For a few milligrams of lyophilized powder the difference is usually too small to see on a barrel, but it's a real approximation and I'd rather say so than pretend the formula is exact. Second, the vial's labeled strength is the strength the calculation trusts. If the label is unclear or the vial doesn't match the paperwork, the arithmetic can't help you and the pharmacy can.

Where this math goes wrong

Almost every error I've seen falls into one of four buckets.

A quick sanity check that catches most of these: convert your unit answer back to mL by dividing by 100, then multiply by the concentration. If you don't land back on your original dose in mg, something in the chain is off.

What the calculator won't decide

It converts numbers you already have. It doesn't know your prescription. Which compound, how much water, and how often are all outside what the arithmetic can see. If you type in a dose, the calculator assumes you got that dose from a clinician who knows your history.

It also stays quiet on anything physical. Storage, handling, how you get the liquid out of a vial and into your body, expiration after mixing: all of that lives in the printed instructions that came with your medication and in the conversation with the person who prescribed it. I built a converter, and a converter is all it is.

If a number surprises you, stop and ask. An unexpected unit count is much more often a mislabeled input than a broken formula, and the two minutes it takes to check with a pharmacist are cheap.

How LockedIn handles this

LockedIn ships this calculator today: enter the vial strength in mg and the bacteriostatic water in mL, and it returns the concentration and the units on a U-100 syringe. It sits next to the GLP-1 tools I built for the same reason, which cover dose logging, titration plans, dose-day reminders, injection site rotation, and doses plotted on the weight trend so you can see them against your weigh-ins. None of it prescribes anything; it records what your clinician already decided and does the arithmetic so you're not doing it on a phone calculator at the kitchen counter. It runs on iPhone and in a browser, and it's free while the app is in beta.

Try it in the browser

Common questions

How much bacteriostatic water do I add to a 5 mg vial?

That volume comes from your prescriber or the manufacturer's instructions, not from a rule of thumb. Arithmetically, 1 mL of bacteriostatic water in a 5 mg vial gives 5 mg/mL and 2 mL gives 2.5 mg/mL. The total milligrams in the vial never change no matter how much water you add.

How do I convert mL to units on a U-100 syringe?

Multiply by 100. A U-100 syringe is calibrated at 100 units per milliliter, so 0.2 mL is 20 units and 0.05 mL is 5 units. If your syringe is U-40 instead, the multiplier is 40.

How many units is 0.5 mg from a 5 mg vial mixed with 2 mL of water?

20 units on a U-100 syringe. The concentration is 5 divided by 2, which is 2.5 mg/mL. Then 0.5 divided by 2.5 is 0.2 mL, and 0.2 times 100 is 20 units.

Does adding more bacteriostatic water weaken the dose?

No. It makes the liquid more dilute, so the same dose takes up more volume and sits at a higher unit mark on the syringe. The milligrams delivered stay identical as long as you recalculate the units for the new concentration.

My dose is written in mcg. How do I use it in the formula?

Divide by 1000 to get milligrams first, so 250 mcg becomes 0.25 mg. Mixing micrograms and milligrams puts you off by a factor of a thousand, which is why the conversion goes first.

Track it instead of guessing at it

LockedIn puts food, GLP-1 doses, workouts and weight in one place. Free while it is in beta.